Coding

Part:BBa_K2275004:Design

Designed by: I-Tai Shih   Group: iGEM17_NCKU_Tainan   (2017-10-09)


Glutamine synthetase (glnA) with reverse glutamate dehydrogenase (gudB)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 449
    Illegal BamHI site found at 1083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 96
    Illegal NgoMIV site found at 862
    Illegal NgoMIV site found at 2228
    Illegal AgeI site found at 352
    Illegal AgeI site found at 2665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1255


Design Notes

None


Source

glnA: Pseudomonas putida gudB: Bacillus subtilis

References